Soskiclab data access committee

Dr Blagoje Soskic
blagoje.soskic@fht.org

Request Access

Dataset ID	Description	Technology	Samples
EGAD50000002113	CRISPR perturbations of IRF4, PRDM1, and AAVS1 (within PPP1R12C gene) in activated B cells	Illumina NovaSeq 6000	96
EGAD50000002114	We developed a B cell stimulation protocol that allowed us to expand a smaller number of naive B cells in culture (2,000 B cells at day 0). On day 6, we performed single-cell RNA-and BCR-sequencing on the entire culture, preserving both clonal diversity and size.	Illumina NovaSeq 6000	32
EGAD50000002115	We purified human naive and memory B cells from PBMCs. We stimulated them with IgM cross-linker providing BCR stimulation, multimeric CD40L that engages costimulatory receptor CD40 on the B cell, and cytokines IL-2 and IL-21. To comprehensively characterise activation dynamics of naive and memory B cells, we performed single-cell RNA sequencing (scRNA-seq) and B cell receptor sequencing (BCR-seq) in resting cells (day 0), before the first division (day 1), at the time of the first division (day 3) and following the full B cell differentiation (day 6)	Illumina NovaSeq 6000	168