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FASTQ files of total RNA-Seq data from the POPS SGA (Small for Gestational Age) samples

All the samples were obtained from the Pregnancy Outcome Prediction–a prospective cohort study of nulliparous women attending the Rosie Hospital, Cambridge (UK) for their dating ultrasound scan between January 14, 2008, and July 31, 2012. Ethical approval for the study was given by the Cambridgeshire 2 Research Ethics Committee (reference number 07/H0308/163) and all participants provided written informed consent. Cases of preeclampsia (PET) were defined on the basis of the 2013 ACOG criteria and cases of small for gestational age (SGA)infants were confined to severe SGA, i.e. a customized birth weight <5th percentile. Chorionic villi from the corresponding placentas (free from decidua, visible infarction, calcification, hematoma, or damage) were collected and processed within 30 minutes of separation from the uterus. After repeated washes in chilled phosphate buffered saline, the samples were placed in RNA later (Applied Biosystems) and stored at -80°C. Total placental RNA was extracted using mirVana Isolation Kit (Ambion). For each placenta, approximately 5 mg of tissue were homogenized in the Lysis/Binding solution for 20 sec at 6 m/s using a bead beater (FastPrep24) and Lysing Matrix D Tubes (MP Biomedicals). The samples were then spun at 13,000 rpm for 5 min at 4°C and the supernatants recovered. Afterwards, the manufacturer's instructions were followed. Immediately after the RNA extraction, placental RNA samples were DNase-treated using DNA-free DNA Removal Kit (Ambion), aliquoted, and stored in -80°C. Quantity and quality of the RNA samples were assessed using the Agilent 2100 Bioanalyzer, the Agilent RNA 6000 Nano Kit (Agilent Technologies), and Qubit fluorometer. Libraries were prepared starting with 300-500 ng of good quality total RNA (RIN ≥7.5) using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina), according to the manufacturer's instructions. The kit contains 96 uniquely indexed adapter combinations in order to allow pooling of multiple samples prior to sequencing. After determining their size (with the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA Kit by Agilent Technologies) and concentration (by qPCR with the KAPA Illumina ABI Prism Library Quantification Kit, Kapa Biosystems), libraries have been pooled and sequenced (single-end, 125 bp) using a Single End V4 Cluster Kit and an Illumina HiSeq2500 or HiSeq4000 instrument.

Request Access

DUO:0000006
version: 2019-01-07

health or medical or biomedical research

This data use permission indicates that use is allowed for health/medical/biomedical purposes; does not include the study of population origins or ancestry.

DUO:0000026
version: 2019-01-07

user specific restriction

This data use modifier indicates that use is limited to use by approved users.

POPS data access policy

You are not authroised to access POPS data unless you have a written permission from the data access committee which you can contact below: D. Stephen Charnock-Jones. Professor of Reproductive Biology. Department of Obstetrics and Gynaecology. University of Cambridge. The Rosie Hospital, Cambridge. CB2 0SW UK email dscj1@cam.ac.uk Tel +44 1223 336875 Fax +44 1223 215327

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001002205 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF00001707733 fq.gz 283.1 MB Report
EGAF00001707734 fq.gz 282.8 MB Report
EGAF00001707735 fq.gz 283.2 MB Report
EGAF00001707736 fq.gz 264.1 MB Report
EGAF00001707737 fq.gz 335.0 MB Report
EGAF00001707738 fq.gz 333.8 MB Report
EGAF00001707739 fq.gz 331.5 MB Report
EGAF00001707740 fq.gz 333.3 MB Report
EGAF00001707741 fq.gz 333.9 MB Report
EGAF00001707742 fq.gz 337.2 MB Report
EGAF00001707743 fq.gz 337.4 MB Report
EGAF00001707744 fq.gz 312.7 MB Report
EGAF00001707745 fq.gz 306.0 MB Report
EGAF00001707746 fq.gz 307.1 MB Report
EGAF00001707747 fq.gz 311.6 MB Report
EGAF00001707748 fq.gz 312.9 MB Report
EGAF00001707749 fq.gz 308.6 MB Report
EGAF00001707750 fq.gz 310.1 MB Report
EGAF00001707751 fq.gz 311.7 MB Report
EGAF00001707752 fq.gz 413.4 MB Report
EGAF00001707753 fq.gz 420.3 MB Report
EGAF00001707754 fq.gz 416.2 MB Report
EGAF00001707755 fq.gz 395.3 MB Report
EGAF00001707756 fq.gz 370.2 MB Report
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EGAF00001707760 fq.gz 371.4 MB Report
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EGAF00001707763 fq.gz 347.1 MB Report
EGAF00001707764 fq.gz 402.6 MB Report
EGAF00001707765 fq.gz 394.6 MB Report
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EGAF00001707768 fq.gz 390.6 MB Report
EGAF00001707769 fq.gz 392.2 MB Report
EGAF00001707770 fq.gz 393.7 MB Report
EGAF00001707771 fq.gz 363.9 MB Report
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EGAF00001707774 fq.gz 342.4 MB Report
EGAF00001707775 fq.gz 356.4 MB Report
EGAF00001707776 fq.gz 352.9 MB Report
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EGAF00001707780 fq.gz 356.1 MB Report
EGAF00001707781 fq.gz 352.7 MB Report
EGAF00001707782 fq.gz 334.9 MB Report
EGAF00001707783 fq.gz 325.6 MB Report
EGAF00001707784 fq.gz 325.0 MB Report
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EGAF00001707790 fq.gz 368.2 MB Report
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EGAF00001707793 fq.gz 358.1 MB Report
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EGAF00001707863 fq.gz 472.1 MB Report
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EGAF00001707875 fq.gz 383.5 MB Report
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EGAF00001707977 fq.gz 456.0 MB Report
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EGAF00001707980 fq.gz 356.2 MB Report
EGAF00001707981 fq.gz 357.6 MB Report
EGAF00001707982 fq.gz 352.9 MB Report
EGAF00001707983 fq.gz 355.0 MB Report
EGAF00001707984 fq.gz 355.6 MB Report
EGAF00001707985 fq.gz 329.8 MB Report
EGAF00001707986 fq.gz 326.3 MB Report
EGAF00001707987 fq.gz 326.5 MB Report
EGAF00001707988 fq.gz 327.0 MB Report
EGAF00001707989 fq.gz 325.9 MB Report
EGAF00001707990 fq.gz 326.3 MB Report
EGAF00001707991 fq.gz 326.3 MB Report
EGAF00001707992 fq.gz 326.5 MB Report
EGAF00001707993 fq.gz 304.5 MB Report
EGAF00001707994 fq.gz 386.6 MB Report
EGAF00001707995 fq.gz 385.4 MB Report
EGAF00001707996 fq.gz 383.9 MB Report
EGAF00001707997 fq.gz 386.1 MB Report
EGAF00001707998 fq.gz 386.5 MB Report
EGAF00001707999 fq.gz 400.3 MB Report
EGAF00001708000 fq.gz 392.6 MB Report
EGAF00001708001 fq.gz 400.3 MB Report
EGAF00001708002 fq.gz 401.0 MB Report
EGAF00001708003 fq.gz 400.1 MB Report
EGAF00001708004 fq.gz 372.1 MB Report
EGAF00001708005 fq.gz 382.6 MB Report
EGAF00001708006 fq.gz 379.5 MB Report
EGAF00001708007 fq.gz 380.8 MB Report
EGAF00001708008 fq.gz 381.0 MB Report
EGAF00001708009 fq.gz 379.2 MB Report
EGAF00001708010 fq.gz 384.5 MB Report
EGAF00001708011 fq.gz 380.5 MB Report
EGAF00001708012 fq.gz 359.7 MB Report
EGAF00001708013 fq.gz 401.2 MB Report
EGAF00001708014 fq.gz 403.4 MB Report
EGAF00001708015 fq.gz 404.0 MB Report
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EGAF00001708017 fq.gz 401.6 MB Report
EGAF00001708075 fq.gz 455.6 MB Report
EGAF00001708076 fq.gz 456.8 MB Report
EGAF00001708077 fq.gz 421.2 MB Report
EGAF00001708078 fq.gz 400.1 MB Report
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EGAF00001708085 fq.gz 373.0 MB Report
EGAF00001708086 fq.gz 473.9 MB Report
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EGAF00001708089 fq.gz 473.5 MB Report
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EGAF00001708091 fq.gz 478.1 MB Report
EGAF00001708092 fq.gz 478.8 MB Report
EGAF00001708093 fq.gz 443.8 MB Report
EGAF00001708094 fq.gz 389.8 MB Report
EGAF00001708095 fq.gz 390.2 MB Report
EGAF00001708096 fq.gz 361.9 MB Report
EGAF00001708097 fq.gz 352.8 MB Report
EGAF00001708098 fq.gz 354.3 MB Report
EGAF00001708099 fq.gz 359.6 MB Report
EGAF00001708100 fq.gz 360.8 MB Report
EGAF00001708101 fq.gz 356.5 MB Report
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EGAF00001708104 fq.gz 332.4 MB Report
EGAF00001708105 fq.gz 370.6 MB Report
EGAF00001708106 fq.gz 369.4 MB Report
EGAF00001708107 fq.gz 369.5 MB Report
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EGAF00001708111 fq.gz 369.3 MB Report
EGAF00001708112 fq.gz 344.3 MB Report
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EGAF00001708120 fq.gz 427.3 MB Report
EGAF00001708121 fq.gz 429.5 MB Report
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EGAF00001708124 fq.gz 426.6 MB Report
EGAF00001708125 fq.gz 421.1 MB Report
EGAF00001708126 fq.gz 421.3 MB Report
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EGAF00001708128 fq.gz 417.2 MB Report
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EGAF00001708131 fq.gz 398.8 MB Report
EGAF00001708189 fq.gz 433.1 MB Report
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