About file location

As the EGA is co-managed by the EMBL-EBI and CRG, files could be located in the UK and/or Spain, as indicated by their respective flags.

Alternatively, files could be located in one of the international Federated EGA nodes, as indicated next to the node's flag.
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RNA-sequencing on thyroid samples from fetuses with Down syndrome and fetuses with no genetic/developmental abnormality

The current data pertains RNA-sequencing reads obtained from thyroid samples acquired from fetuses with Down syndrome and fetuses with no genetic/developmental abnormality. Total RNA was isolated from left lobe from thyroid samples using a hand-held homogenizer and the Promega ReliaPrep RNA Miniprep System (Thermo Fisher Scientific). RNA yield was determined with the NanoDrop Microvolume Spectrophotometer (Thermo Fisher Scientific). Fragmentation and mRNA library preparation was performed using the Kapa mRNA Hyperprep Kit (Roche, Basel, Switzerland). Libraries were equimolar pooled and quality was checked on a TapeStation system using the DNA1000 ScreenTape (Agilent Technologies, Santa Clara, CA, USA). Libraries were sequenced with poly(A) selection to sequence all messenger RNA for gene expression analysis on the NovaSeq6000 PE150 (Illumina, San Diego, CA, USA), producing at least 40M 150-bp paired-end reads per library.

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Data Access Policy for the study "Multi-omic analysis of thyroid dysfunction in Down Syndrome"

Access requests undergo a comprehensive review by the Data Access Committee (DAC) for the study "Multi-omic analysis of thyroid dysfunction in Down Syndrome" as performed at the Amsterdam UMC. Request will only be granted to qualified users for appropriate use. Applicants are required to submit detailed proposals outlining the purpose, methodology, and ethical considerations of their research. Procedure: 1) The Applicant sends a data access request through European Genome-phenome Archive (EGA) to the DAC. 2) Upon submission, a representative of the DAC will send a Data Request Form (DRF) to the Application. Upon completing the DRF, this form must be returned to the DAC whereupon the evaluation process will commence. 3) When a request is positively evaluated, the applicant will be asked to sign the Data Sharing Agreement. Review and Conditions: - Each DRF is assessed based on the scientific profile of the applicants, the scientific merit, ethical implications, data security plans, and compliance with the relevant regulations, within a specified timeframe. - The DAC reserves the right to conduct periodic reviews to ensure responsible data use. - Approval is contingent on meeting the criteria and compliance with relevant regulations.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000000264 RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
i
EGAF50000093227 fastq.gz 1.4 GB Report
EGAF50000093228 fastq.gz 1.3 GB Report
EGAF50000093229 fastq.gz 2.3 GB Report
EGAF50000093230 fastq.gz 2.2 GB Report
EGAF50000093231 fastq.gz 1.3 GB Report
EGAF50000093232 fastq.gz 1.3 GB Report
EGAF50000093233 fastq.gz 2.4 GB Report
EGAF50000093234 fastq.gz 2.5 GB Report
EGAF50000093235 fastq.gz 1.5 GB Report
EGAF50000093236 fastq.gz 1.5 GB Report
EGAF50000093237 fastq.gz 2.6 GB Report
EGAF50000093238 fastq.gz 2.5 GB Report
EGAF50000093239 fastq.gz 1.5 GB Report
EGAF50000093240 fastq.gz 1.5 GB Report
EGAF50000093241 fastq.gz 2.7 GB Report
EGAF50000093242 fastq.gz 2.6 GB Report
EGAF50000093243 fastq.gz 1.5 GB Report
EGAF50000093244 fastq.gz 1.5 GB Report
EGAF50000093245 fastq.gz 2.6 GB Report
EGAF50000093246 fastq.gz 2.7 GB Report
EGAF50000093247 fastq.gz 1.4 GB Report
EGAF50000093248 fastq.gz 1.4 GB Report
EGAF50000093249 fastq.gz 2.5 GB Report
EGAF50000093250 fastq.gz 2.6 GB Report
EGAF50000093251 fastq.gz 1.5 GB Report
EGAF50000093252 fastq.gz 1.5 GB Report
EGAF50000093253 fastq.gz 2.5 GB Report
EGAF50000093254 fastq.gz 2.5 GB Report
EGAF50000093255 fastq.gz 2.4 GB Report
EGAF50000093256 fastq.gz 2.4 GB Report
EGAF50000093257 fastq.gz 3.9 GB Report
EGAF50000093258 fastq.gz 4.0 GB Report
EGAF50000093259 fastq.gz 1.7 GB Report
EGAF50000093260 fastq.gz 1.7 GB Report
EGAF50000093261 fastq.gz 2.9 GB Report
EGAF50000093262 fastq.gz 3.0 GB Report
EGAF50000093263 fastq.gz 1.2 GB Report
EGAF50000093264 fastq.gz 1.2 GB Report
EGAF50000093265 fastq.gz 2.2 GB Report
EGAF50000093266 fastq.gz 2.1 GB Report
EGAF50000093267 fastq.gz 1.6 GB Report
EGAF50000093268 fastq.gz 1.6 GB Report
EGAF50000093269 fastq.gz 2.7 GB Report
EGAF50000093270 fastq.gz 2.7 GB Report
EGAF50000093271 fastq.gz 1.5 GB Report
EGAF50000093272 fastq.gz 1.6 GB Report
EGAF50000093273 fastq.gz 2.6 GB Report
EGAF50000093274 fastq.gz 2.7 GB Report
48 Files (101.4 GB)