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Dataset contains all Single cell RNAseq data of human neurons from the study Bouwen et al Nat Comm 2025

These documents contain the patch seq data presented in the Bouwen et al Nat Comm 2025. Human brain samples were gathered during surgery, neurons were isolated using patch clamp glass pipette. Isolated cells were processed using Smartseq2 pipeline, and sequenced using an Illumina 2500. Files are formatted in fastq format. There are a total of 12 patient samples, each with 2 replication runs.

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Data access agreement

============================================================================================================= This agreement governs the terms on which access will be granted to the phenotype and genotype data generated by the Gao Lab, Tumor associated epilepsy project. ============================================================================================================= Gao Lab, Tumor associated epilepsy project DATA ACCESS AGREEMENT This agreement governs the terms on which access will be granted to the  phenotype and genotype data generated by the Gao Lab, Tumor associated epilepsy project. In signing this agreement, You are agreeing to be bound by the terms and  conditions of access set out in this agreement. For the sake of clarity, the terms of access set out in this agreement  apply both to the User and the User’s Institution (as defined below).  User Institution and User are referred to within the agreement as “You”  and “Your” shall be construed accordingly. Definitions: Project means the Gao Lab, Tumor associated epilepsy project comprised out of the following  members:  Dr. Zhenyu Gao and Dr. Bibi Bouwen Data means all and any human genetic/transcriptomic data and matching metadata obtained from the Project. Data Subject means a person, who has been informed of the purpose for  which the Data is held and has given his/her informed consent thereto. User means a researcher whose User Institution has previously completed  this Data Access Agreement and has received acknowledgement of its  acceptance. Publications means, without limitation, articles published in print  journals, electronic journals, reviews, books, posters and other written  and verbal presentations of research. User Institution means the organization at which the User is employed,  affiliated or enrolled. Terms and Conditions: In signing this Agreement: 1. You agree to use the Data only for the advancement of medical  research, according to the consent obtained from sample donors. 2. You agree not to use the data from this cohort or any part thereof  for the creation of products for sale or for any commercial purpose. 3. You agree to preserve, at all times, the confidentiality of  information and Data pertaining to Data Subjects. In particular, You  undertake not to use, or attempt to use the Data to compromise or  otherwise infringe the confidentiality of information on Data Subjects  and their right to privacy. 4. You agree not to attempt to link the data provided under this  agreement to other information or archive data available for the data  sets provided, even if access to that data has been formally granted to  you, or it is freely available without restriction, without specific  permission being sought from the relevant access committees. 5. You agree not to transfer or disclose the Data, in whole or part, or  any identifiable material derived from the Data, to others, except as  necessary for data/safety monitoring or programme management. Should You  wish to share the Data with a collaborator within the same Institution,  the third party must make a separate application for access to the Data. 6. You agree to use the data for the approved purpose and project  described in your application; use of the data for a new purpose or  project will require a new application and approval. The relevant access  committees will see to it that the Data is not used in publications  outside the project described in the original application. 7. You accept that Data will be reissued from time to time, with  suitable versioning. If the reissue is at the request of sample donors  and/or other ethical scrutiny, You will destroy earlier versions of the  Data. 8. You agree to abide by the terms outlined in the Project  'Publications Policy' as set out in Schedule 1. 9. You agree to acknowledge in any work based in whole or part on the  Data, the published paper from which the Data derives, the version of  the data, and the role of the Project and the relevant primary  collectors and their funders. Suitable wording is provided in the  Publications Policy given in Schedule 1. 10. You accept that the Project, the original data creators,  depositors or copyright holders, or the funders of the Data or any part  of the Data supplied: a) bear no legal responsibility for the accuracy  or comprehensiveness of the Data; and b) accept no liability for  indirect, consequential, or incidental, damages or losses arising from  use of the Data, or from the unavailability of, or break in access to,  the Data for whatever reason. 11. You understand and acknowledge that the Data is protected by  copyright and other intellectual property rights, and that duplication,  except as reasonably required to carry out Your research with the Data,  or sale of all or part of the Data on any media is not permitted. 12. You recognize that nothing in this agreement shall operate to  transfer to the User Institution any intellectual property rights  relating to the Data. The User Institution has the right to develop  intellectual property based on comparisons with their own data. 13. You accept that this agreement will terminate immediately upon any  breach of this agreement by You and You will be required to destroy any  Data held. 14. You accept that it may be necessary for the Project or its  appointed agent to alter the terms of this agreement from time to time  in order to address new concerns. In this event, the Project or its  appointed agent will contact You to inform You of any changes and You  agree that Your continued use of the Data shall be dependent on the  parties entering into a new version of the Agreement. 15. You agree that you will submit a report to the Project Data  Access Committee, if requested, on completion of the agreed purpose. The  Project Data Access Committee agrees to treat the report and all  information, data, results, and conclusions contained within such report  as confidential information belonging to the User Institution. 16. You accept that the Data is protected by and subject to  international laws, and that You are responsible for ensuring compliance  with any such applicable law. The Project Data Access Committee  reserves the right to request and inspect data security and management  documentation to ensure the adequacy of data protection measures in  countries that have no national laws comparable to that which pertain in  the EAA. 17. This agreement shall be construed, interpreted and governed by the  laws of the Netherlands and shall be subject to the non-exclusive  jurisdiction of the Dutch courts. SCHEDULE 1 Publications Policy The primary purpose of the project is to accelerate efforts to identify genome/transcriptome sequence variants in in brain tumors and find covariates related to differentiation, recurrence and therapy, through implementation and analysis of large-scale genome-wide association studies. Additional objectives include the development and validation of informatics and analytical solutions appropriate to the scale and nature of the project, as well as use of the data generated to answer important methodological  and biological questions relevant to association studies in general. The Project anticipates that data generated from the project will be  used by others, such as required for developing new analytical methods,  in understanding patterns of expression, structural variants, polymorphism and in guiding selection of markers to map genes involved in specific diseases. Authors who use data from the project must acknowledge the Project  using the following wording "This study makes use of data generated for  the Bouwen-Gao project. Users should note that the Project bears no responsibility for the  further analysis or interpretation of these data, over and above that  published by the Project. =============================================================================================================

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001369 Transcriptome Sequencing

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF50000480856 fastq.gz 787.7 MB
EGAF50000480857 fastq.gz 331.5 MB
EGAF50000480858 fastq.gz 643.5 MB
EGAF50000480859 fastq.gz 677.6 MB
EGAF50000480860 fastq.gz 282.0 MB
EGAF50000480861 fastq.gz 276.7 MB
EGAF50000480862 fastq.gz 283.1 MB
EGAF50000480863 fastq.gz 330.7 MB
EGAF50000480864 fastq.gz 274.3 MB
EGAF50000480865 fastq.gz 420.6 MB
EGAF50000480866 fastq.gz 425.6 MB
EGAF50000480867 fastq.gz 427.3 MB
EGAF50000480868 fastq.gz 638.9 MB
EGAF50000480869 fastq.gz 450.2 MB
EGAF50000480870 fastq.gz 436.1 MB
EGAF50000480871 fastq.gz 436.9 MB
EGAF50000480872 fastq.gz 420.3 MB
EGAF50000480873 fastq.gz 421.5 MB
EGAF50000480874 fastq.gz 812.3 MB
EGAF50000480875 fastq.gz 671.8 MB
EGAF50000480876 fastq.gz 440.2 MB
EGAF50000480877 fastq.gz 288.6 MB
EGAF50000480878 fastq.gz 280.7 MB
EGAF50000480879 fastq.gz 422.5 MB
24 Files (10.9 GB)