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Single-cell RNA sequencing of metastatic colorectal cancer organoids treated with cetuximab

Organoids were seeded in 12-well plates at a density of 5 × 10⁴ cells per well, embedded in 200 µL of Matrigel diluted 1:1 with culture medium, and maintained under EGF-deprived conditions. After four days of growth, organoids were either left untreated or treated with cetuximab (20 µg/mL). Following 72 hours of treatment, organoids were harvested, dissociated, and live single cells were sorted for single-cell RNA sequencing using the 10x Genomics platform.

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DUO:0000007
version: 2021-02-23

disease specific research

This data use permission indicates that use is allowed provided it is related to the specified disease.

ModifiersMONDO:0005575

General policy for IRCC datasets

Translational Cancer Medicine laboratory of Candiolo IRCC institute. Dataset available on request

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001460 Transcriptome Sequencing

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF50000507997 fastq.gz 566.0 MB
EGAF50000507998 fastq.gz 544.7 MB
EGAF50000507999 fastq.gz 1.9 GB
EGAF50000508000 fastq.gz 4.8 GB
EGAF50000508001 fastq.gz 937.7 MB
EGAF50000508002 fastq.gz 942.3 MB
EGAF50000508003 fastq.gz 3.1 GB
EGAF50000508004 fastq.gz 7.4 GB
EGAF50000508005 fastq.gz 1.8 GB
EGAF50000508006 fastq.gz 6.6 GB
EGAF50000508007 fastq.gz 16.2 GB
EGAF50000508008 fastq.gz 1.0 GB
EGAF50000508009 fastq.gz 1.0 GB
EGAF50000508010 fastq.gz 3.6 GB
EGAF50000508011 fastq.gz 8.7 GB
EGAF50000508012 fastq.gz 1.1 GB
EGAF50000508013 fastq.gz 1.2 GB
EGAF50000508014 fastq.gz 3.8 GB
EGAF50000508015 fastq.gz 9.2 GB
EGAF50000508016 fastq.gz 2.8 GB
EGAF50000508017 fastq.gz 6.6 GB
EGAF50000508018 fastq.gz 2.7 GB
EGAF50000508019 fastq.gz 6.3 GB
EGAF50000508020 fastq.gz 2.7 GB
EGAF50000508021 fastq.gz 6.2 GB
25 Files (101.8 GB)