Single-cell RNA sequencing of metastatic colorectal cancer organoids treated with cetuximab
Organoids were seeded in 12-well plates at a density of 5 × 10⁴ cells per well, embedded in 200 µL of Matrigel diluted 1:1 with culture medium, and maintained under EGF-deprived conditions. After four days of growth, organoids were either left untreated or treated with cetuximab (20 µg/mL). Following 72 hours of treatment, organoids were harvested, dissociated, and live single cells were sorted for single-cell RNA sequencing using the 10x Genomics platform.
- 10/12/2025
- 8 samples
- DAC: EGAC50000000068
- Technology: unspecified
DUO:0000007 version: 2021-02-23
disease specific research
This data use permission indicates that use is allowed provided it is related to the specified disease.
ModifiersMONDO:0005575
General policy for IRCC datasets
Translational Cancer Medicine laboratory of Candiolo IRCC institute. Dataset available on request
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS50000001460 | Transcriptome Sequencing |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Quality Report |
Located in
i
|
|---|---|---|---|---|
| EGAF50000507997 | fastq.gz | 566.0 MB |
|
|
| EGAF50000507998 | fastq.gz | 544.7 MB |
|
|
| EGAF50000507999 | fastq.gz | 1.9 GB |
|
|
| EGAF50000508000 | fastq.gz | 4.8 GB |
|
|
| EGAF50000508001 | fastq.gz | 937.7 MB |
|
|
| EGAF50000508002 | fastq.gz | 942.3 MB |
|
|
| EGAF50000508003 | fastq.gz | 3.1 GB |
|
|
| EGAF50000508004 | fastq.gz | 7.4 GB |
|
|
| EGAF50000508005 | fastq.gz | 1.8 GB |
|
|
| EGAF50000508006 | fastq.gz | 6.6 GB |
|
|
| EGAF50000508007 | fastq.gz | 16.2 GB |
|
|
| EGAF50000508008 | fastq.gz | 1.0 GB |
|
|
| EGAF50000508009 | fastq.gz | 1.0 GB |
|
|
| EGAF50000508010 | fastq.gz | 3.6 GB |
|
|
| EGAF50000508011 | fastq.gz | 8.7 GB |
|
|
| EGAF50000508012 | fastq.gz | 1.1 GB |
|
|
| EGAF50000508013 | fastq.gz | 1.2 GB |
|
|
| EGAF50000508014 | fastq.gz | 3.8 GB |
|
|
| EGAF50000508015 | fastq.gz | 9.2 GB |
|
|
| EGAF50000508016 | fastq.gz | 2.8 GB |
|
|
| EGAF50000508017 | fastq.gz | 6.6 GB |
|
|
| EGAF50000508018 | fastq.gz | 2.7 GB |
|
|
| EGAF50000508019 | fastq.gz | 6.3 GB |
|
|
| EGAF50000508020 | fastq.gz | 2.7 GB |
|
|
| EGAF50000508021 | fastq.gz | 6.2 GB |
|
|
| 25 Files (101.8 GB) | ||||
