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Single cell RNAseq FASTQ files of three PDAC organoid lines (P28, P40, P47) using SORT-seq

This dataset contains raw FASTQ files from single cell RNA sequencing of three patient-derived pancreatic ductal adenocarcinoma (PDAC) organoid lines (P28, P40, P47) cultured under standard conditions. For each line, single cells were FACS sorted into 384-well capture plates, with each well containing a 50nl droplet of barcoded primers. Plates were processed following an adapted SORT-seq protocol, and cDNA libraries were generated using CEL-Seq2 with TruSeq small RNA primers (Illumina). Sequencing was performed on an Illumina NextSeq500 platform using paired-end reads (read 1: 26 cycles, index read: 6 cycles, read 2: 60 cycles). Data are controlled-access and intended for single cell transcriptomic analysis of PDAC organoid heterogeneity and WNT pathway activity.

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DUO:0000006
version: 2021-02-23

health or medical or biomedical research

This data use permission indicates that use is allowed for health/medical/biomedical purposes; does not include the study of population origins or ancestry.

Data Access Policy for Maurice Lab

Data access is restricted to bona fide researchers for research purposes only. Access requests will be reviewed by the Data Access Committee. Applicants must agree to the Data Access Agreement, including keeping the data secure and not sharing it with unauthorized third parties.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS50000001544 RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Quality Report
Located in
EGAF50000558985 fastq.gz 553.9 MB
EGAF50000558986 fastq.gz 552.6 MB
EGAF50000558987 fastq.gz 247.2 MB
EGAF50000558988 fastq.gz 694.8 MB
EGAF50000559005 fastq.gz 246.7 MB
EGAF50000559006 fastq.gz 555.6 MB
EGAF50000559007 fastq.gz 307.0 MB
EGAF50000559008 fastq.gz 245.4 MB
EGAF50000559009 fastq.gz 307.0 MB
EGAF50000559010 fastq.gz 689.9 MB
EGAF50000559011 fastq.gz 247.1 MB
EGAF50000559012 fastq.gz 556.1 MB
EGAF50000559013 fastq.gz 307.6 MB
EGAF50000559014 fastq.gz 304.7 MB
EGAF50000559060 fastq.gz 315.6 MB
EGAF50000559061 fastq.gz 701.0 MB
EGAF50000559062 fastq.gz 314.0 MB
EGAF50000559063 fastq.gz 696.8 MB
EGAF50000559064 fastq.gz 688.7 MB
EGAF50000559065 fastq.gz 316.7 MB
EGAF50000559071 fastq.gz 694.1 MB
EGAF50000559099 fastq.gz 699.9 MB
EGAF50000559100 fastq.gz 316.3 MB
EGAF50000559101 fastq.gz 701.6 MB
24 Files (11.3 GB)