Single-cell RNA-seq profiling of patient derived organoids

The aim of this study was to profile developement of resistance to cancer treatments using organoids. Two patient derived colorectal cancer organoids were profiled using single cell RNAseq after treatment with a combination of different anticancer drugs. The organoids were profiled both after 4 weeks of treatment and after a drug holiday of 2 weeks.

Type: RNASeq
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 1 Publication

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000001495	This dataset consists of single-cell RNA-seq profiling of colorectal cancer organoids derived from two patients. The organoids were profiled both after 4 weeks of treatment and after a drug holiday of two weeks. There are 49 RNA sequence files generated using single cell RNA sequencing technology from 10X genomics.	Illumina NovaSeq 6000	49

Publications	Citations
Epigenetic Heritability of Cell Plasticity Drives Cancer Drug Resistance through a One-to-Many Genotype-to-Phenotype Paradigm. Oliveira EA, Milite S, Fernandez-Mateos J, Cresswell GD, Yara-Romero E, Vlachogiannis G, Chen B, James CT, Patruno L, Ascolani G, Acar A, Heide T, Spiteri I, Graudenzi A, Caravagna G, Bertotti A, Graham TA, Magnani L, Valeri N, Sottoriva A. Cancer Res 85: 2025 2921-2938	2