CLUSTER RNAseq Study of healthy control cohort

CLUSTER aims to discover novel disease mechanisms and biomarkers in children suffering from Juvenile idiopathic arthritis (JIA). Healthy controls were used as the control for baseline expression compared to children with JIA. The samples used in this study comes from blood samples that were collected from healthy volunteers recruited through Centre for Adolescent Rheumatology Versus Arthritis, spanning from children to adult age. These samples were then processed for PBMC isolation followed by sorting cells into 4 different immune cell types. All of these samples were then sequenced by NovaSeq6000,together with the JIA samples.

Type: RNASeq
Archiver: European Genome-Phenome Archive (EGA)

5 Datasets

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD50000002073	This is the RNAseq PBMC dataset (paired FASTQ files, n = 37 paired files) generated from healthy donor cohort samples. Demographic dataset is also included in this dataset. Samples were sequenced on the NovaSeq6000 instrument (Illumina, San Diego, US) at 300pM, using a 100bp paired read run with corresponding 8bp dual sample index and 5bp or 8bp unique molecular index reads. Run data were demultiplexed and converted to FASTQ files using Illumina’s BCL Convert Software v3.75.	Illumina NovaSeq 6000	172
EGAD50000002074	This is the RNAseq CD8 dataset (paired FASTQ files, n = 36 paired files) generated from healthy donor cohort samples. CD8 cells were sorted from isolated total PBMC by cell sorter (BD FACSAriaTM III, BD Biosciences). Demographic dataset is also included in this dataset. Samples were sequenced on the NovaSeq6000 instrument (Illumina, San Diego, US) at 300pM, using a 100bp paired read run with corresponding 8bp dual sample index and 5bp or 8bp unique molecular index reads. Run data were demultiplexed and converted to FASTQ files using Illumina’s BCL Convert Software v3.75.	Illumina NovaSeq 6000	172
EGAD50000002075	This is the RNAseq CD4 dataset (paired FASTQ files, n = 37 paired files) generated from healthy donor cohort samples. CD4 cells were sorted from isolated total PBMC by cell sorter (BD FACSAriaTM III, BD Biosciences). Demographic dataset is also included in this dataset. Samples were sequenced on the NovaSeq6000 instrument (Illumina, San Diego, US) at 300pM, using a 100bp paired read run with corresponding 8bp dual sample index and 5bp or 8bp unique molecular index reads. Run data were demultiplexed and converted to FASTQ files using Illumina’s BCL Convert Software v3.75.	Illumina NovaSeq 6000	172
EGAD50000002076	This is the RNAseq CD19 dataset (paired FASTQ files, n = 31 paired files) generated from healthy donor cohort samples. CD19 cells were sorted from isolated total PBMC by cell sorter (BD FACSAriaTM III, BD Biosciences). Demographic dataset is also included in this dataset. Samples were sequenced on the NovaSeq6000 instrument (Illumina, San Diego, US) at 300pM, using a 100bp paired read run with corresponding 8bp dual sample index and 5bp or 8bp unique molecular index reads. Run data were demultiplexed and converted to FASTQ files using Illumina’s BCL Convert Software v3.75.	Illumina NovaSeq 6000	172
EGAD50000002077	This is the RNAseq CD14 dataset (paired FASTQ files, n = 31 paired files) generated from healthy donor cohort samples. CD14 cells were sorted from isolated total PBMC by cell sorter (BD FACSAriaTM III, BD Biosciences). Demographic dataset is also included in this dataset. Samples were sequenced on the NovaSeq6000 instrument (Illumina, San Diego, US) at 300pM, using a 100bp paired read run with corresponding 8bp dual sample index and 5bp or 8bp unique molecular index reads. Run data were demultiplexed and converted to FASTQ files using Illumina’s BCL Convert Software v3.75.	Illumina NovaSeq 6000	172